What is a suction head? What is a good suction tip? How to choose a suction head?
-This is the common perception of almost all users about the suitability of a suction head. This statement can be said to be partly but not entirely true.
It is true that a pipette tip that can be mounted on a pipette can be pipetted with the pipette in a pipetting system, but is this reliable? There is a question mark here. The answer to this question requires data.
1. You may wish to test the performance of a pipette with matching tips. Rinse the tip and perform several repetitions of the dosing operation, weigh each dosing volume, and record the reading.
2. Calculate the accuracy and precision of the pipetting operation after converting the density of the test liquid into volume.
3、What we need to choose is a pipette tip with good accuracy. If the pipette and the pipette tip are matched with poor accuracy, it means that the sealing of the tip and the pipette is not guaranteed, so that the results of each operation are not reproducible.
So what are the minimum requirements for a good pipette tip?
A good pipette tip depends on concentricity, taper and, more importantly, adsorption;
1. Let's start with the taper: if it is better, the match with the gun will be very good, and the suction will be more accurate;
2. Concentricity: concentricity is the tip of the suction nozzle and the tip and pipette link at the circle vertically seems to be the same center, if it is not the same center of the circle means that the concentricity is not good;
3. Then come to the more important one is our adsorption: adsorption and the material of the tip, if the material of the tip is not good, it will affect the accuracy of pipetting, resulting in a large amount of liquid retention or referred to as wall, resulting in pipetting errors;
So we should pay special attention to the above three points when choosing a pipette tip.
Bad pipette tips put a row of obviously different spacing it! Will see a very obvious distortion, which is a very important step in choosing whether it is a good pipette tip.