Product Details
MeltonVEC™ Sendai Virus iPSC Reprogramming Kit can efficiently induce somatic cells into induced pluripotent stem cells (iPSCs), making the reprogramming process more stable and controllable. The kit is suitable for various starting cell types, including peripheral blood mononuclear cells (PBMCs) and T cells, and can be used to establish iPSC lines with different genetic backgrounds.
The MeltonVEC™ Sendai Virus Reprogramming Kit is an efficient, non-integrating iPSC reprogramming product suitable for various starting cell types. It balances reprogramming efficiency, operational convenience, and the advantage of rapid vector clearance during cell line establishment.
Key features include:
Efficient reprogramming of various starting cells, including fibroblasts, blood cells, and others.
Specially optimized for PBMC reprogramming, further improving reprogramming efficiency and shortening the induction period.
Rapid clearance of RNA vectors, facilitating subsequent cell applications.
Sendai virus vectors are suitable for iPSC reprogramming of various somatic cell types.
| The following somatic cell types have been successfully used for iPSC induction. | ||
| adult and neonatal dermal fibroblasts | amniotic fluid MSCs | cardiac fibroblasts |
| CD34-positive blood cells | mammary epithelial cells | nasal epithelial cells |
| peripheral blood mononuclear cells (PBMCs) | skeletal muscle myoblasts | T cells |
| umbilical vein epithelial cells | urothelial cells | |
Description of specifications for the MeltonVEC™ Sendai Virus iPSC Reprogramming Kit
| MeltonVEC™ Sendai Virus iPSC Reprogramming Kit | |
| Description | non-integrating RNA virus |
| reprogramming efficiency | 0.05-1% |
| non-genomic integration | Yes |
| blood cell reprogramming | Yes, it is applicable to a variety of cell types (CD34+ cells, PBMCs, and T cells) with high efficiency. |
| Special equipment requirements | None |
| reprogramming factors | Oct4, Sox2, Klf4, and c-Myc |
| kit specifications | Catalog Number: MSV1001-2 (Fibroblast version) |
Component specifications: 3 tubes in total, 60 µL per tube. | |
| KOS (Klf4, Oct3/4, and Sox2) | |
| c-Myc | |
| Klf4 | |
| Residual reprogramming vector detection | qPCR |
| Storage conditions | -80℃ |
| Shelf life | 12 months |
Introduction to the general workflow of peripheral blood mononuclear cell (PBMC) reprogramming:
Practical advice:
iPS reprogramming can be optimized by varying the amount of Klf4 to achieve maximal reprogramming efficiency. Generally, the multiplicity of infection (MOI) of KOS and c-Myc vectors should be kept at a 1:1 ratio, while the Klf4 vector can be varied independently. The standard MOI ratio for KOS:c-Myc:Klf4 is 5:5:3. To improve reprogramming efficiency, this can be changed to 5:5:6 or 10:10:6.
Images of test results shared by some customers are shown below:
